American Society of Hematology

Figure 2

$Figure 2. Flow cytometric and morphologic analyses of cells. (A) Typical flow cytometric analyses of cells in BM, spleen, and thymus. Cells were analyzed for expression of the indicated molecules. Typical data are presented for one control mouse that received a transplant with GFP-only–transduced cells and 3 mice that received a transplant with ERG-transduced cells. The “a,“ “c,” “e,” and “f” correspond to mice shown in Figure 1G. (B) Cytospin preparations of spleen cells in an ERG mouse (f) showing clusters of immature erythroid (left) and lymphoid (right) cells. Original magnifications: ×1000 (SPlan100 100×/1.25 oil 160/0.17 objective). (C) Flow cytometric analysis of DNA content of indicated cells in mice that received a transplant with control and ERG-transduced BM cells. Percentage of S phase is presented. Ter-119+ cells were flow-sorted before the analysis. (D-E) Analyses of granulocyte/macrophage, megakaryocytic, and B-cell compartment in ERG mice. Cells obtained from BM and spleen were gated on GFP+ and GFP− fractions for flow cytometric analysis of the indicated molecules. Typical data of 10 mice analyzed are presented (D). Lineage-negative BM cells of control and ERG mice were assayed for colony formation under a granulocyte/macrophage condition, in triplicate. Mean and SD are presented for a typical analysis of 3 independent experiments (E).$

Flow cytometric and morphologic analyses of cells. (A) Typical flow cytometric analyses of cells in BM, spleen, and thymus. Cells were analyzed for expression of the indicated molecules. Typical data are presented for one control mouse that received a transplant with GFP-only–transduced cells and 3 mice that received a transplant with ERG-transduced cells. The “a,“ “c,” “e,” and “f” correspond to mice shown in Figure 1G. (B) Cytospin preparations of spleen cells in an ERG mouse (f) showing clusters of immature erythroid (left) and lymphoid (right) cells. Original magnifications: ×1000 (SPlan100 100×/1.25 oil 160/0.17 objective). (C) Flow cytometric analysis of DNA content of indicated cells in mice that received a transplant with control and ERG-transduced BM cells. Percentage of S phase is presented. Ter-119⁺ cells were flow-sorted before the analysis. (D-E) Analyses of granulocyte/macrophage, megakaryocytic, and B-cell compartment in ERG mice. Cells obtained from BM and spleen were gated on GFP⁺ and GFP⁻ fractions for flow cytometric analysis of the indicated molecules. Typical data of 10 mice analyzed are presented (D). Lineage-negative BM cells of control and ERG mice were assayed for colony formation under a granulocyte/macrophage condition, in triplicate. Mean and SD are presented for a typical analysis of 3 independent experiments (E).

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