Tie2-cre–mediated loss of Zeb2 causes intracranial hemorrhaging at E12.5. (A) Zeb2^{−/ΔTie2-Cre} hemorrhagic phenotype. (B) Hematoxylin/eosin staining of E12.5 Zeb2^{−/ΔTie2-Cre} embryo (200× magnification) showing bleeding especially in the germinal layer and ganglia/ganglionic eminences of the developing brain. GE indicates ganglionic eminence; and G, ganglion.