Figure 1
Figure 1. DMOG increases BMDAC viability by decreasing apoptosis and increasing proliferation. (A) Equal numbers of BMDACs were cultured in endothelial growth media in the presence of vehicle or DMOG for 4 days, and viable cells were counted using the Trypan blue exclusion method. *P < .001, DMOG vs vehicle. #P < .001 vs time 0; Bonferroni post hoc test after 2-way ANOVA. The overall effects of time and DMOG treatment on BMDAC survival were significant (P < .001). (B) Apoptosis was detected at day 4 as phycoerythrin-conjugated annexin V (annexin V-PE) positive/7-amino-actinomycin D (7-AAD) negative cells (green rectangle) using flow cytometry. (C) Cell cycle distribution of BMDACs stained with propidium iodide and analyzed by flow cytometry. Data are mean ± SEM (n = 3 or 4).

DMOG increases BMDAC viability by decreasing apoptosis and increasing proliferation. (A) Equal numbers of BMDACs were cultured in endothelial growth media in the presence of vehicle or DMOG for 4 days, and viable cells were counted using the Trypan blue exclusion method. *P < .001, DMOG vs vehicle. #P < .001 vs time 0; Bonferroni post hoc test after 2-way ANOVA. The overall effects of time and DMOG treatment on BMDAC survival were significant (P < .001). (B) Apoptosis was detected at day 4 as phycoerythrin-conjugated annexin V (annexin V-PE) positive/7-amino-actinomycin D (7-AAD) negative cells (green rectangle) using flow cytometry. (C) Cell cycle distribution of BMDACs stained with propidium iodide and analyzed by flow cytometry. Data are mean ± SEM (n = 3 or 4).

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