HIV-specific CD8+ T cells from ECs express higher amounts of T-bet than CPs after short-term stimulation. (A) The fraction of Gag- or Nef-specific CD8+ T cells, as identified by staining for IFN-γ, TNFα, or CD107a, that fell within the T-betbright gate was determined for all ECs, CPs, and HAART subjects. (B) Representative flow cytometric plots from ECs and CPs showing the fraction of the Nef-specific response that fell within the 3 T-bet gates. Events shown have been gated on CD8+ T cells. (C) The fraction of CMV-specific CD8+ T cells that fell within the T-betbright gate was determined for all ECs, CPs, and HAART subjects as in panel A. (A-C) Statistical analysis was carried out using 1-way ANOVA (nonparametric; Kruskal-Wallis) followed by a Dunn test for multiple comparisons. *P < .05; **P < .01. Bars represent the means and error bars indicate SDs.
