New and preexisting vasculature is leaky in kindlin-2^+/− mice. (A) Fibrin content (brown) in tumors grown in WT and kindlin-2^+/− mice. Scale bars, 30 μm. (B) Quantification of fibrin-positive area shown in panel A. (C) Representative photographs of necrotic areas in tumors grown in WT and kindlin-2^+/− mice. Sections were stained with hematoxylin and eosin. Scale bars, 200 μm (top panel) and 150 μm (bottom panel). (D) Quantification of necrotic areas showed increased necrosis in tumors grown in kindlin-2^+/− mice. All data are mean ± SEM and are representative of 3 independent experiments, including 5-8 mice per group as indicated. (E) Comparison of leakage from ear blood vessels in WT and kindlin-2^+/− mice on stimulation with mustard oil. Blue ears of kindlin-2^+/− mice indicate enhanced leakage of Evans Blue dye from blood vessels (left panel). Quantitative analysis revealed increased leakage from ear blood vessels in kindlin-2^+/− mice compared with WT mice (right panel). (F) Representative photographs of Evans blue leakage from dorsal skin vasculature of WT and kindlin-2^+/− mice on application of PBS or VEGF (left panel). Quantification of dorsal skin vasculature permeability (right panel). All vascular permeability data are expressed as mean ± SEM and are representative of 3 independent experiments with 5-6 mice per group as indicated.