Abnormal angiogenesis in kindlin-2^+/− mice. (A) Representative images of tumor sections stained with CD31 Ab (brown; left panel). Scale bars, 200 μm (top panel), 50 μm (bottom panel). Image analysis shows decreased area of CD31-stained vasculature in tumors grown in kindlin-2^+/− mice compared with WT mice (right panel). Data are representative of 3 independent experiments with 6 mice per group. (B) Average weight of prostate tumors grown in kindlin-2^+/− mice is lower than in WT mice (left panel) and representative tumors are shown (right panel). Data are means ± SEM, (n = 7 mice per group) and are representative of 3 independent experiments. (C) Angiogenesis in Matrigel implants in the presence of VEGF. (Left panel) Hemoglobin content in Matrigel implants from kindlin-2^+/− mice was higher compared with WT mice. (Right panel) Representative images of Matrigel implant sections stained with CD31 Ab (brown). Scale bars, 150 μm. All data are representative of 3 independent experiments including 6 mice per group. CD31-positive areas were quantified in 5-10 independent fields per tumor implant or Matrigel plug and the average area per field was determined from duplicate measurements of each of the fields analyzed.