Figure 3
![Figure 3. Direct effect of NAADP on [Ca2+]i mobilization. (A) Representative plot of NAADP-AM–induced Ca2+ mobilization in EA.hy926 cells. (B) Increase in F/F0 in cells stimulated with NAADP-AM compared with cells stimulated with vehicle alone (control). Cells were stimulated with 100μM TMPH after microinjection with (C) vehicle alone or (D) inactivating concentrations of NAADP. Increase in F/F0 in response to TMPH (E) and 2 U/mL of thrombin (F) in cells microinjected with the inactivating concentration of 500μM NAADP compared with cells microinjected with vehicle alone. Where applicable, error bars represent SEM; n = 3 independent experiments; *P < .05 by Student t test.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/117/18/10.1182_blood-2010-02-266338/4/zh89991169790003.jpeg?Expires=1725429195&Signature=wDjXdj0F7qcguUGaCzWftLxqzY~MVRZvtsCdFZJMl7Xv08~ghR0Wfi6KuVQ1PHve0z7a0LONkGIDHFM2VNw-wQAaYZgW26v6uA3roaeKJhgEfGvCOqRvEiqm6qXMTp-WT~FJacpZnOvXJlIwB8PJvNL~EvQsIecaf6nlk9Ff73fOtnvEMJI4TLd87U9FFr7BX4qL-B3UqupTsRNvQGj6LEWOOmq~EaFVP~QyoOx9AKEVUGrL7dX6nl9uWFhlwMxb4RKTH7Pl-EVfvw8Jdhbk7Uf-6GCqa5G8XhTUY47jQZwY3ZcO5Gkt~pAlgTybshmZjtZVmG4wwJ8sqqg3Vpcp8A__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Direct effect of NAADP on [Ca2+]i mobilization. (A) Representative plot of NAADP-AM–induced Ca2+ mobilization in EA.hy926 cells. (B) Increase in F/F0 in cells stimulated with NAADP-AM compared with cells stimulated with vehicle alone (control). Cells were stimulated with 100μM TMPH after microinjection with (C) vehicle alone or (D) inactivating concentrations of NAADP. Increase in F/F0 in response to TMPH (E) and 2 U/mL of thrombin (F) in cells microinjected with the inactivating concentration of 500μM NAADP compared with cells microinjected with vehicle alone. Where applicable, error bars represent SEM; n = 3 independent experiments; *P < .05 by Student t test.
