Figure 2
Adhesion of FKN-coated microspheres to platelets immobilized in μ-slides. Leukocyte-free platelets were immobilized in μ-slides. After removal of nonadherent platelets, surface-attached platelets were incubated with a CX3CR1 neutralizing antibody (anti-CX3CR1), matched isocontrol (control IgG), or vehicle alone (untreated) before perfusion with FKN-coated microspheres (FKN) or BSA-coated microspheres (albumin) suspended in PBS for 15 minutes at a wall shear rate of 150 seconds. After washing the μ-slides with PBS, the number of adherent microspheres was counted. Results are the mean ± SEM of 3 independent experiments with platelets from different leukocyte concentrates. ***P < .001 in 1-way analysis of variance with Bonferroni Multiple Comparison Test versus the number of adherent control microspheres perfused over identically treated platelets.

Adhesion of FKN-coated microspheres to platelets immobilized in μ-slides. Leukocyte-free platelets were immobilized in μ-slides. After removal of nonadherent platelets, surface-attached platelets were incubated with a CX3CR1 neutralizing antibody (anti-CX3CR1), matched isocontrol (control IgG), or vehicle alone (untreated) before perfusion with FKN-coated microspheres (FKN) or BSA-coated microspheres (albumin) suspended in PBS for 15 minutes at a wall shear rate of 150 seconds. After washing the μ-slides with PBS, the number of adherent microspheres was counted. Results are the mean ± SEM of 3 independent experiments with platelets from different leukocyte concentrates. ***P < .001 in 1-way analysis of variance with Bonferroni Multiple Comparison Test versus the number of adherent control microspheres perfused over identically treated platelets.

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