Release of sGPVI from platelets induced by coagulation in human plasma. Coagulation in citrated PRP was induced by recalcification at 37°C in the absence (A-B) or presence (C-D) of added TF plus phospholipids. Thrombin generation was measured using the calibrated automated thrombin assay (A,C). sGPVI was measured by ELISA, after addition of 10mM ethylenediaminetetraacetic acid and centrifugation for removal of platelets or the platelet-rich fibrin clot to generate serum (B,D). Data are representative of 3 independent experiments using PRP from 3 separate donors.