In vivo G-CSF therapy increases neutrophil 2-DG uptake, the expression of GLUT1, and intracellular G6P, lactate, and ATP levels. BM neutrophils were isolated from 6- to 8-week-old control (+/+) and G6pc3^−/− (−/−) littermates after 5-day G-CSF therapy. (A) Neutrophil 2-DG uptake in untreated and 5-day G-CSF–treated control and G6pc3^−/− mice. Data are the mean ± SEM of 3 (untreated) or 6 (G-CSF–treated) independent experiments. (B) Representative immunofluorescence of neutrophil GLUT1 staining (green fluorescence), pan Cadherin membrane staining (red fluorescence), and DAPI nuclei staining (blue fluorescence) (original magnification ×1000). (C) Neutrophil G6P, lactate, and ATP levels in untreated and 5-day G-CSF–treated control and G6pc3^−/− mice. Data are the mean ± SEM of 3 (untreated) or 6 (G-CSF–treated) independent experiments. (D) Quantitative flow cytometric analysis of survival of in vitro cultured neutrophils isolated from 5-day G-CSF–treated wild-type (○, ●) and G6pc3^−/− (Δ, ▴) mice in the absence (○, Δ) or presence (●, ▴) of G-CSF. Data are the mean ± SEM of 4 independent experiments. **P < .005. *P < .05.