Effects of limiting glucose, fMLP, C5a, CytB, and 2-DG on apoptotic death of G6pc3^−/− BM neutrophils cultured in vitro. The annexin V–depleted BM neutrophils were isolated from 6- to 8-week-old unaffected (+/+) and G6pc3^−/− (−/−) littermates as described in “Neutrophil isolation.” (A) Quantitative analysis of neutrophil survival in untreated wild-type (○, ●, □, ■) and G6pc3^−/− (Δ, ▴, ▿, ▾) mice in 6.2mM glucose (○, ●, Δ, ▴,) or 0.6mM glucose (□, ■, ▿, ▾)-containing medium in the absence (○, □, Δ, ▿) or presence (●, ■, ▴, ▾) of G-CSF. Data are the mean ± SEM of 4 independent experiments. (B) Effect of fMLP, C5a, or CytB on neutrophil 2-DG uptake. Glucose uptake was examined in annexin V–depleted BM neutrophils that were treated at 37°C for 15 minutes with 10⁻⁶M fMLP, 0.8 μg/mL C5a, or 5 μg/mL CytB. Data are the mean ± SEM of 3 independent experiments. (C) Quantitative analysis of neutrophil survival in untreated wild-type (○, ×, □, ●) and G6pc3^−/− (Δ, ▿, ★, ▴) mice in 6.2mM glucose-containing medium in the presence of 10⁻⁶M fMLP (×, ▿), 0.8 μg/mL C5a (□, ★), or 5 μg/mL CytB (●, ▴). (D) Quantitative analysis of neutrophil survival in untreated wild-type (○, ●, □, ■) and G6pc3^−/− (Δ, ▴, ▿, ▾) mice in 6.2mM glucose-containing medium in the absence (○, Δ, ●, ▴) or presence of 1mM 2-DG (□, ■, ▿, ▾), in the presence of G-CSF (●, ▴), or 2-DG/G-CSF (■, ▾). **P < .005. *P < .05.