The ER stress and cell survival signaling pathways in G6pc3^−/− BM neutrophils. BM neutrophils were isolated from 6- to 8-week-old control (+/+) and G6pc3^−/− (−/−) littermates as described in “Neutrophil isolation.” (A) Western blot analysis of protein extracts of neutrophils using antibodies against p-PERK, eIF2α, p-eIF2α, ATF4, CHOP, or β-actin. Each lane contains 50 μg protein. The relative protein levels of p-PERK, p-eIF2α, ATF4, or CHOP were quantified by densitometry of 3 or 4 separate pairs of Western blots. (B) Western blot analysis of protein extracts of neutrophils using antibodies against p-Akt, total Akt, p-SHIP1, SHIP1, PI3K-p85α, PI3K-p110α PTEN, p-PTEN, or β-actin. Each lane contains 50 μg protein. The relative protein levels of p-Akt or p-SHIP1 were quantified by densitometry of 3 or 4 separate pairs of Western blots. (C-D) Flow cytometric analysis of p-Akt (C) or PtdIns(3,4,5)P₃ (D). Data are the mean ± SEM of 4 independent experiments. **P < .005. *P < .05.