Influence of antioxidant therapy on time to arterial thrombosis occlusion. WT (n = 6) and PRCP^gt/gt (n = 8) mice were left untreated or treated with the antioxidants apocynin (Apo; n = 6 WT; n = 11 PRCP^gt/gt; A) or tempol (n = 6 WT; n = 5 PRCP^gt/gt; B) for 7 days. At the conclusion of the treatment period, carotid artery occlusion times were performed using the Rose-Bengal carotid artery thrombosis model. One-way analysis of variance was used to determine the difference among groups. Data presented are the mean ± SEM of ≥ 5 mice for each condition. (C) Hydrogen peroxide (H₂O₂) levels were measured by Amplex Red in renal tissue harvested from WT (n = 7), PRCP^gt/gt (n = 9), or PRCP^gt/gt + tempol-treated (n = 5) mice. (D) Constitutive aortic expression of endothelial cell mRNA for KLF2, KLF4, eNOS, or thrombomodulin (TM) in WT (n = 3) or PRCP^gt/gt (n = 4) mice. *P < .05.