The influence of PRCP on arterial thrombosis risk. (A) Control C57BL/6 WT and PRCP^gt/gt (gt/gt) mice were subjected to the Rose-Bengal and ferric chloride carotid artery thrombosis occlusion models. Data are expressed as the time to complete arterial occlusion. A 1-way analysis of variance was used to determine differences among groups. Data represent the mean ± SEM of 10 WT and 12 PRCP^gt/gt mice for the Rose Bengal assay and 4 WT and 5 PRCP^gt/gt mice for the ferric chloride assay. (B) The time to carotid artery occlusion on the Rose Bengal assay was determined in C57BL/6 mice that were untreated (WT, open bar graph, n = 13) or treated with the inhibitor to the PRCP, ZPP (n = 4), or plasma kallikrein inhibitors SBTI (n = 4), PFRCK (n = 5), or PKSI 527 (PKSI; n = 6). Data represent the mean ± SEM of 4 to 13 animals in each group. (C) Determination of the IC₅₀ of PFRCK on 2nM plasma kallikrein or factor XIIa. Inhibition studies were performed as indicated in supplemental Methods. Data represent the mean ± SEM of 3 reactions. (D) Determination of the IC₅₀ of SBTI on 5nM plasma kallikrein or factor XIa. Inhibition studies were performed as indicated in supplemental Methods. Data represent the mean ± SEM of 3 reactions. *P < .05.