Sema7A is cleaved and shed during platelet activation. (A-B) Shed sema7A in platelet supernatants with increasing time after (A) 10μM PMA or (B) 10μM SFLLRN activation. (C-D) Surface expression of sema7A determined by flow cytometry after platelet activation with (C) PMA or (D) SFLLRN activation. Alexa Fluor-488–conjugated goat IgG was used as the isotype control. (E-F) Representative Western blots of sema7A on platelet membranes.