cAng-1 increases HIF-1α translation through mTOR activation. RT-PCR of HIF-1α (A) in a hypoxia time-dependent (n = 3) or (B) in a dose-dependent cAng-1 treatment (n = 3). (C-D) HUVECs treated with hypoxia or hypoxia/cAng-1 (200 ng/mL) for 6 hours followed by treatment with 15μM cyclohexamide (CHX) or 10μM MG132. Quantitative graph from 2 independent experiments express HIF-1α levels on normalization for α-tubulin. (E) Western blotting of mTOR cascade. cAng-1 augmented hypoxia-induced acute activation of Akt and mTOR phosphorylation (n = 3). Vertical lines have been inserted to indicate a repositioned gel lane.