Mechanisms regulating age-related methylation changes. (A) Venn diagram showing overlapping hypomethylated markers in granulocytes, monocytes, and mPB-HPCs. (B) Correlation of genes showing age-related methylation changes with known PRC2 targets. (C) Transcription factor binding motif analysis of hypermethylated promoters in mPB-HPCs was performed as in Figure 4D and compared with the hypomethylated core dataset of differentiated myeloid cell populations (Figure 4D). A substantial difference in the transcription factor binding profiles for differentiation- and age-related methylation changes was observed.