Mechanisms regulating differentiation-dependent demethylation. (A) Ingenuity Pathway Analysis (IPA) of markers with differentiation-dependent demethylation. (B) Percentage of genes showing differentiation-dependent methylation changes in CpG islands (CGIs). (C) Association of genes showing differentiation-dependent methylation changes with known PRC2 targets. (D) Analysis of hypomethylated promoters in differentiated myeloid cell populations for specific transcription factor binding motifs with the use of PScan and transcription factor-binding motifs from the JASPAR database (see “Methods”). A specific set of transcription factor binding shows a high probability of binding near promoters of genes derived from the hypomethylated core set. (E) Motifs of transcription factors involved in hematopoiesis are highly enriched near promoters derived from the hypomethylated core set.