Analysis of differentiation-dependent methylation changes in CB-HPCs. Comparison of genomic DNA methylation profiles of granulocytes (A) and monocytes (B) with those of CB-HPCs. Red dots indicate differentially methylated sites. (C) Venn diagram showing an overlapping set of 490 hypomethylated CpG dinucleotides (core set). (D) Validation of selected markers by 454 bisulfite sequencing. Schematic outline of validation genes. Gray lines represent the sequenced regions, arrows indicate transcriptional start sites, vertical lines represent individual CpG dinucleotides, and triangles indicate CpG markers interrogated on the array. Sequencing results are shown as heatmaps in which each row represents one sequence read. Individual red boxes indicate methylated and green boxes indicate unmethylated CpG dinucleotides. Sequencing gaps are shown in white. Panels below heatmaps summarize the results from 454 bisulfite sequencing (454) and from the array-based methylation analysis (Inf). Sequencing coverage ranged from 74 to 1644 reads, as indicated.