Ly49G2⁺ NK cells key to priming MCMV-specific CD8 T-cell response. (A) Representative dot plots showing the proportion of M45-D^b–specific CD8 T cells in spleens of uninfected R7, infected R7 treated with control rat IgG, infected R7 depleted of NK cells (PK136) and infected R7 depleted of Ly49G2⁺ NK cells (4D11). The dot plots were gated on CD19⁻, CD3⁺ cells. (B) The graph represents the mean ± SEM for live gated M45-D^b–specific CD8 T cells in uninfected and infected R7 animals given the indicated treatments. (C) The graph represents the mean ± SEM for live gated M139-K^b–specific CD8 T cells in uninfected and infected R7 animals given the indicated treatments. The data are representative of 4 experiments with 3-4 mice per treatment group (**P < .005, ***P < .0005 by Student t test compared with rat IgG treated).