Effect of MHC I D^k virus resistance on MCMV-specific CD8 T-cell response. Control and MCMV-infected R7 and R2 splenocytes were stained for CD19, CD3, CD8, and virus-specific T-cell receptors using M45-D^b tetramers before flow cytometric analysis. (A) The graph represents the mean ± SEM for live gated CD8⁺ T cells (CD19⁻, CD3⁺) in uninfected and infected spleens from R7 and R2 mice. (B) Representative dot plots showing the proportion of M45-D^b–specific CD8 T cells in uninfected and infected R7 and R2 spleens. The dot plots were gated on CD19⁻, CD3⁺ cells. (C) The graph represents the mean ± SEM for virus-specific CD8 T cells in uninfected and infected R7 and R2 spleens. (D) The graph represents the mean ± SEM for virus-specific CD8 T cells in blood collected from serially bled R7 animals. (A-C) The data are representative of 4 experiments with 4 mice per strain per time point. (D) The data are representative of 2 experiments with 4 mice per strain per time point (*P < .005, **P < .001, ***P < .0005, by Student t test).