MHC I D^k is sufficient to support splenic DCs after MCMV infection. (A) Representative dot plots showing the proportion of CD11c^hi, MHC II⁺ cDCs in uninfected and infected Tg-D^k and non-Tg spleens. The dot plots were gated as described for Figure 1. (B) Representative dot plots showing the proportion of CD8α⁺ and CD11b⁺ cDC subsets in uninfected and infected Tg-D^k and non-Tg spleens. The dot plots were gated on CD3⁻, CD19⁻, CD11c^hi, MHC II⁺ cells. (C) The graph represents the mean ± SEM for live gated cDC subsets in Tg-D^k spleen. (D) The graph represents the mean ± SEM for live gated cDC subsets in non-Tg spleen. The data are representative of 4 experiments with 4 mice per strain per time point. Both CD8α⁺ and CD11b⁺ cDC subsets were significantly higher on day 3.5 in Tg-D^k than non-Tg mice (P < .001), whereas cDC subsets were comparable in uninfected animals (*P < .05, **P < .001 compared with respective subset in uninfected mice by Student t test).