ARC19499 effect on thrombin generation in human plasma. (A-C) Left panels show lag time; middle panels, ETP; right panels, peak thrombin. Thrombin generation was initiated with 1pM TF. In all graphs, the effect of ARC19499 (♦) was compared with the negative control oligonucleotide (■). Lines represent the activity of normal plasma (solid lines) and factor-deficient plasma (dashed lines) in the absence of aptamer. (A) Activity in hemophilia A plasma. (B) Activity in inhibitor plasma. (C) Activity in hemophilia B plasma. Results are represented as the means ± SEM. Shaded areas around the solid and dashed lines represent the SEM for the normal and factor-deficient plasma, respectively, in the absence of aptamer; n = 3 for each plasma.