ARC17480 and ARC19499 binding to TFPI and other proteins. (A-C) Representative curves showing binding between radiolabeled ARC17480 and TFPI, as well as activated coagulation proteins (A), protease inhibitors (B), and coagulation zymogens (C). The percentage of protein-bound aptamer is plotted as a function of protein concentration. (D) Competition-binding experiments using 10nM TFPI and radiolabeled ARC17480, with unlabeled ARC17480, ARC19499, or control oligonucleotide as competitors. The percentage of protein-bound radiolabeled aptamer is plotted as a function of the unlabeled competitor concentration. Representative curves are shown.