PCFT is essential for intestinal folate uptake and tissue accumulation. (A) In vivo uptake of tritiated folic acid to the indicated tissues was measured at the time points shown. (B) Uptake of folate and zinc protoporphyrin (ZnPP) by HEK293 cells transiently transfected with vector only or with mouse or human PCFT, as indicated, was measured. Data are presented as in Figure 2. *P < .05.