Confirmation of log2 ratio imbalance among chromosomes. The manner in which the log2 ratio imbalance occurred was confirmed. (A) SP-49 showed no imbalance. Log2 ratios of gain regions were 0.55 (arrowhead and dotted line). Log2 ratios of loss regions were −0.88 (arrowhead and dotted line). (B) HANK1 showed no imbalance. Log2 ratios of gain regions were 0.55 (arrowhead and dotted line). (C) Mixed-genomic DNA at a ratio of 7:3 reproduced the log2 ratio imbalance. The log2 ratio of chromosome 2p14-pter of the mixed DNA sample was 0.55 (arrowhead). Chromosome 2p had a copy region identical to both SP-49 and HANK1. Arrows indicate the log2 ratio imbalance.