Increased percentage donor chimerism in MHC-matched transplant recipients. The percentage of GFP+ cells within the myeloid and lymphoid gates was plotted for each individual transplant recipient. Each recipient animal received 50 to 75 × 103 WKM cells in addition to 2 × 105 peripheral blood carrier cells. Each unique symbol represents a single transplant recipient. Sixteen recipients were evaluated 16 weeks after transplantation, including all the unmatched recipients. Five of the matched recipients were evaluated at 14 weeks. The red lines indicate the thresholds for myeloid (> 4%) and lymphoid (> 0.6%) engraftment. Mean percentage of GFP+ cells ± SEM is shown for the engrafted animals in each group. The level of donor chimerism in the engrafted matched recipients was significantly higher than for the engrafted unmatched recipients (P = .0002 and P = .05 for the myeloid and lymphoid gates, respectively).
