RUNX3 mediates the effects of CXCL12 in CD4 and CD14 expression. (A) PBMos were freshly isolated and cultivated in the presence (+) or absence ⁽⁻⁾ of AMD3100 (25 μg/mL) or CCX733 (100nM) for 48 hours. Membrane CD4 was analyzed by flow cytometry. Results are expressed as percentages of positive cells. Mean ± SD; n = 4; *P < .05. (B) THP-1 cells were transfected with RUNX3 siRNA or control siRNA. The expression of CD4 and CD14 was evaluated by flow cytometry the third day. Mean ± SD; n = 4; *P < .05.