Gal1 expression in EBV-transformed LCLs and primary EBV⁺ PTLDs. (A) Gal1 expression in a cHL cell line (L428), a series of EBV-transformed LCLs (NOR-, RIC-, STA-, FOL-, LOV-, RIV-, WOL-, FW-, VS-, MA-, SC-, DS-, and DW-LCL), and a DLBCL cell line (SU-DHL6). (B) Gal1 immunohistochemical staining of 3 representative primary EBV⁺ PTLDs (i-iii) and a DLBCL (iv). In subpanel iv, a Gal1⁺ macrophage is indicated with a red arrow and representative Gal1⁻ DLBCL tumor cells are indicated with black arrows. The recently developed murine αGal1 mAb 8F4F8G7 was used at 1:20 000 (final concentration, 250 ng/mL) for immunoblots (A) and at 1:40 000 (final concentration, 125 ng/mL) for immunohistochemistry (B). Original magnifications are 1000×.