Representative patient. (A) Coronary angiogram showing a lesion in the mid left anterior descending artery. (B) Two-dimensional quantitative coronary angiography showing 52% DS (arrow). (C) Three-dimensional quantitative coronary angiography showing cross-sectional area stenosis (CS) of 62% and lesion length of 12.25 mm. (D) Shear stress map generated from flow field calculated by computational fluid dynamics analysis, showing peak wall shear stress of 33.1 Pa. (E-G) Flow cytometry density plot of platelet CD62P expression in the coronary artery proximal (E) and distal (F) to the lesion, and in the coronary sinus (G). (H) Histogram overlay of platelet CD62 expression in the 3 sites. MFI indicates mean fluorescence intensity.