C5a inhibits RhoA activation during phagocytosis by neutrophils, by a PI3Kδ-dependent effect. (A) C5a inhibits the activation of neutrophil RhoA in response to opsonized zymosan, and preincubation with PI3K inhibitor IC87114 prevents this inhibition. P = .005 by Kruskal-Wallis ANOVA; *P < .05, **P < .01 by Dunn posthoc test. Data presented as median and interquartile ranges (n = 10 healthy donors). (B) The effect of rhC5a on phagocytosis is prevented by preincubation with the PI3Kδ inhibitor IC87114 at 180nM (IC₅₀ for PI3Kδ). P = .008 by Kruskal-Wallis ANOVA; *P < .05 by Dunn posthoc test. Data are shown as median and interquartile range (n = 5 healthy volunteers). (C) The effect of rhC5a on phagocytosis is not prevented by preincubation with the PI3Kγ inhibitor AS605240 at 8nM (IC₅₀ for PI3Kγ). P = .02 by Kruskal-Wallis ANOVA; *P < .05, **P < .01 by Dunn posthoc test. Data are shown as median and interquartile range (n = 5 healthy volunteers). (D) Incubating neutrophils with the PI3KIA (α, β, δ) activator 740Y-P inhibits phagocytosis. P = .01 by Kruskal-Wallis ANOVA; *P < .05, **P < .01 by Dunn posthoc test. Data are shown as median and interquartile range (n = 5 healthy volunteers).