CSN5 has an effect on ROS-mediated deneddylated/neddylated Cul3. (A) Immunoblot analysis of Cul3 from 293 cells infected with Lm for 180 minutes (top) or treated with hydrogen peroxide (H₂O₂) for 120 minutes (bottom). (B) Immunoblot analysis of Cul3 from 293 cells infected with Lm (MOI, 10) or treated with H₂O₂ (1mM) in the presence or absence of diphenylene iodonium chloride (DPI; 40 μM) or NAC (20 mM) 30 minutes before the treatments. (D) Immunoblot analysis of Cul3 from 293 cells transfected with scrambled siRNA or CSN5 (100 nM) for 24 hours and subsequently treated with H₂O₂ for 120 minutes. (D) Immunoblot analysis of Cul3 from CSN5^fl/fl or CSN5^fl/flLyzsM-Cre BMDMs treated with H₂O₂ for 80 minutes. Data are representative of 5 (A-D) experiments.