CSN5 inhibits the expression of antioxidation and anti-inflammation genes regulated by NRF2. (A) Western blot analysis of the production of HO-1 protein in CSN5^fl/fl or CSN5^fl/flLyzsM-Cre macrophages activated with LPS (100 ng/mL) or poly(I:C) (50 μg/mL) for 24 hours. An α-tubulin blot was included to indicate lane loading. Data are representative of 5 experiments. (B) Immunoblot analysis of HO-1 lysates from CSN5^fl/flLyzsM-Cre primary peritoneal macrophages transfected with scrambled siRNA or mouse HO-1 siRNA and Western blot analyzed with anti–HO-1 antibodies. An α-tubulin blot was included to indicate lane loading. (C) ELISA of TNF-α production in macrophages transfected with scrambled siRNA or HO-1 siRNA for 24 hours and subsequently activated with poly(I:C) (50 μg/mL) for an additional 24 hours. (D) ELISA of TNF-α production in macrophages treated with poly(I:C) (50 μg/mL), ZnPP (1 μM), ZnPP plus poly(I:C), CuPP (1 μM), or CuPP plus poly(I:C) for 24 hours. Data are representative of 3 experiments or the mean ± SEM of 4 experiments (C-D).