TLR ligand and TNF-α but not IL-1β induce activation of MAPKs Erk and p38 that depend on CSN5. Immunoblot analysis of phosphorylated p38 and phosphorylated Erk from CSN5^fl/fl and CSN5^fl/flLyzsM-Cre BMDMs stimulated with poly (I:C) (50 μg/mL) for 60 minutes (A), stimulated with LPS (100 ng/mL) for 30 minutes (B). An α-tubulin blot was included to indicate lane loading. Data are representative of ≥ 5 independent experiments. (C) Immunoblot analysis of p38, Erk, and IκBα from CSN5^fl/fl or CSN5^fl/flLyzsM-Cre BMDMs stimulated with TNF-α (30 ng/mL) for 15 minutes. Data are representative of ≥ 3 experiments.