KO of myeloid CSN5 leads to enhanced survival after CLP. (A) Survival after 7 days of moderate CLP or sham CLP in 8- to 12-week-old female or male wild-type littermates (CSN5^fl/fl, n = 25) mice. Data were pooled from the 2 experiments performed. (B) Bacterial CFUs in lung, blood, and liver 48 hours after CLP. (C) ELISA analysis of plasma TNF-α of littermates or CSN5^fl/flLyzsM-Cre mice 8 hours after CLP. (D) Flow cytometric analysis of CD11b⁺Gr-1⁺ cells in the peritoneal fluid of littermates or CSN5^fl/flLyzsM-Cre mice 24 hours after CLP. Data are expressed as mean ± SEM; *P < .05; **P < .01. (E) Survival after 7 days of LPS-challenged wild-type littermates (CSN5^fl/fl n = 50) or CSN5^fl/flLyzsM-Cre (n = 55). Data were pooled from the 3 experiments performed. *P < .05; **P < .01.