Figure 5
![Figure 5. Half-life determination of FXa activity in hemophilic plasma. Factor Xa (A, 20nM; wt-FXa, [■]; or B, FXaI16L [●] and FXaV17A [▴]) were incubated at 25°C in HA plasma. At various time points aliquots were removed and diluted to 0.1 nM in assay buffer. Residual FXa activity was determined using an aPTT clotting assay as detailed in the Methods. The solid lines were drawn following analysis of all data sets to an exponential decay function; estimated half-lives are given in the text. The data are representative of 2 to 3 similar experiments.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/117/1/10.1182_blood-2010-08-300756/4/zh89991062820005.jpeg?Expires=1722386731&Signature=UyVVxt0YK1J3Eglu9Y0FJ~B6fU184J0zwAY3E4pLdrSUJelRgs8vVZgpaQwavN8hDyAGHUnEAtl0rcFokSxTZcvucKuoGfqme-0IfUNnweJ3Y5jEduU0J6o8XyYU9eY737nIxvxBMWwZhCHGMzg3zrpzKM6e5DHIy38BJm5PL1qnilhS7ogA8dIzCvoBVvwx4LiJIIVJLW7w1VA2JpIHbcoqrCTjfzvJHTJUnyUd1EPqBqLGVjMmiZplhnBGQB3LAx22LqqIquXYZG6u6p~OeDA0Y~BCuQhsGbnPPyoCwAW3JwrRB8ZrqrBYWjuLj4oaElO9Dz~sVFIsN2T5NOG7iQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Half-life determination of FXa activity in hemophilic plasma. Factor Xa (A, 20nM; wt-FXa, [■]; or B, FXaI16L [●] and FXaV17A [▴]) were incubated at 25°C in HA plasma. At various time points aliquots were removed and diluted to 0.1 nM in assay buffer. Residual FXa activity was determined using an aPTT clotting assay as detailed in the Methods. The solid lines were drawn following analysis of all data sets to an exponential decay function; estimated half-lives are given in the text. The data are representative of 2 to 3 similar experiments.
