Analysis of ADAMTS13 digestion of full-length VWF and VWF115. Recombinant ADAMTS13 digests were performed as outlined under “In vitro ADAMTS13 digests of recombinant VWF.” (A-B) Varying concentrations of ADAMTS13 were incubated with 1 U/mL full-length VWF for 24 hours with 1.5M Urea. Multimer graphs for wild-type, Y1584C, and R1205H VWF were plotted using a 4-parameter curve fit. The concentration of ADAMTS13 required to cause a 50% loss of multimer height was determined. Symbols represent mean values and SE from 2 experiments. (C-D) Comparison of the ADAMTS13 concentrations necessary for 50% loss of intact human and mouse VWF115. Varying ADAMTS13 concentrations were incubated with VWF115 for 4 hours under nondenaturing conditions. Bars represent the mean values, and each circle represents a separate assay.