CD70-CAR generation, cell-surface expression, and transduction of human T cells. (A) CD70-CAR was generated by fusing full-length CD27 to the signaling domain of the CD3-ζ chain, an IRES sequence, and tCD19 was included for the detection of genetically modified T cells. (B) 293T cells transfected with CD70-CAR constructs express both CD27 and the marker gene tCD19. (C) CD70-CAR expression on transduced human T cells was 45% ± 6%, as determined by staining tCD19. (D) Both CD4 and CD8 T cells were genetically modified.