mTOR and the GR differentially regulate the activation of NF-κB in monocytes. Human monocytes were treated as indicated. After 8 hours of stimulation, (A) IL-12p40 and (B) IL-10 mRNA were assessed by reverse-transcribed polymerase chain reaction. mRNA levels are depicted as increase over the unstimulated controls and represent the mean ± SEM of 3 donors. (C) THP-1 and (D) Tsc2^−/− cells were transfected with an NF-κB luciferase reporter plasmid and treated as indicated. Luciferase activity is shown as the mean ± SEM of 3 independent experiments performed in triplicate. (E) THP-1 cells were transfected with a Gal4 luciferase reporter and an expression plasmid encoding a p65(TAD)-Gal4 fusion. Luciferase activity is shown as the mean ± SEM of 3 independent experiments performed in triplicate. *P < .05. n.s. indicates not significant.