Assessment of vascular leakage and leukocyte infiltration in endothelial-restricted BMPR-II–deficient mice. (A) To assess vascular leakage, L1cre(+)BMPR2f/f mice or L1cre(−)BMPR2f/f mice were injected with SU5416 or vehicle subcutaneously for 23 hours. Evans Blue dye was then administered intravenously via the tail vein for 1 hour, after which the lungs were flushed with PBS and the heart/lung block excised. Evans blue was extracted from the right lung, quantified, and expressed as nanograms of Evans Blue per milligram of dry lung tissue. Data are the mean ± SEM for 7 L1cre(+)BMPR2f/f and 9 L1cre(−)BMPR2f/f mice. In panel A, ANOVA showed a significant effect of treatment with SU5416 and genotype P < .05. *P < .05, **P < .01, compared with L1cre(−)BMPR2f/f animals. (B) To assess leukocyte infiltration, the concentration of MPO in the right lung of L1cre(+)BMPR2f/f and L1cre(−)BMPR2f/f mice was determined. In panel B, ANOVA showed a significant effect of genotype P < .001. **P < .01, ***P < .001, compared with L1cre(−)BMPR2f/f animals.
