Effect of CXCR2 antagonist SB265610 on neutrophil transmigration through HPAEC with reduced BMPR-II expression. Mock-transfected HPAECs or HPAECs transfected with siRNA-targeting BMPR-II or nontargeting control siRNA were seeded into Ibidi IV flow slides, then unstimulated (A), stimulated with TNF-α for 4 hours (B), or TGF-β1 for 24 hours (C). Neutrophils were incubated with the CXCR2 antagonist, SB265610, or vehicle at 37°C for 10 minutes before perfusion across the endothelial monolayer for 2 minutes, followed by washout. Data are the mean ± SEM for 6 independent experiments for unstimulated HPAECs and 3 experiments for TNF-α and TGF-β1–stimulated HPAECs. *P < .05, compared with cells treated with vehicle by Student t test.