Transduction with C/EBPα-C^m alone induced AML in a mouse BMT model. (A) Kaplan-Meier analysis for the survival of mice that received transplants of BM cells transduced with C/EBPα-C^m-IG (C^m, n = 17), C/EBPα-N^m-IG (N^m, n = 8), or mock (pMYs-IG, n = 8). (B) Cytospin preparations of BM cells derived from mice/C/EBPα-C^m (left) or mice/N^m (right) were stained with Giemsa. A representative photograph is shown. Images were obtained with a BX51 microscope and a DP12 camera (Olympus); objective lens, UplanFl (Olympus); original magnification ×100. (C) Flow cytometric analysis of BM cells derived from mice/C/EBPα-C^m (middle), mice/C/EBPα-N^m (right), or mice/pMYs-IG (left). The dot plots show Ly5.1, Gr-1, CD11b, c-kit, B220, or CD19 labeled with phycoerythrin-conjugated monoclonal Ab versus expression of GFP. (D) Expression of C/EBPα-C^m protein and p30 protein generated by C/EBPα-N^m in spleen cells of mice/pMYs-IG (lane 1) and mice/C^m (lanes 2-4) (top) or in spleen cells of mice/pMYs-IG (lane 1) and mice/N^m (lanes 2-4) (bottom). Cell lysates were subject to immunoblotting with anti-C/EBPα (14AA) Ab or anti-tubulin Ab as control. Data are representative of 3 independent experiments. (E-F) Real-time PCR for G-CSF-R (E) or c-Myc (F) in BM cells derived from mice/C^m or mice/pMYs-IG. Expression levels were normalized by β-actin mRNA. The relative expression level of BM derived from mice/mock (lane 1) was defined as 1. All data points correspond to the mean and the standard deviation (SD) of 3 independent experiments. Lanes 1-2: mice/pMYs-IG; lanes 3-6: mice/C^m.