CD73 discriminates afferent and efferent lymphatics. Double stainings of human lymph nodes with anti-CD73 and anti–LYVE-1 antibodies (A) and anti-CD73 and anti-podoplanin antibodies (B). Examples of afferent lymphatics (arrows), endothelium of subcapsular sinuses (open arrows) and HEV (yellow arrows) are pointed out in the appropriate panels. Lymphatic sinuses are indicated with arrowheads. The direction of the lymph node center is indicated with double arrowheads. Scale bars, 100 μm.