Only a subpopulation of lymphatic and blood vessels express CD73. Double immunofluorescence stainings of human skin and gut with anti-CD73 and anti-podoplanin antibodies (recognizing lymphatic vessels; A), with anti-CD73 and anti–LYVE-1 antibodies (recognizing lymphatic vessels; B) and skin, gut, and lymph nodes with CD73 and anti–PV-1 antibodies (recognizing blood vessels; C). (D) Summary of the results in different organs. Podoplanin-, LYVE-1–, and PV-1–positive vessels have been set as 100% by definition and CD73-positive vessels of those have been presented as mean percentages ± SEM; 35-269 vessels/sample were counted depending on the size of the sample. Arrows point to some double-positive vessels. Scale bar, 100 μm.