Sox6 transduction of cord blood–derived CD34⁺ cells causes early loss of progenitor cells. (A) CD34⁺ cells transduced with Sox6 grew more slowly than EV-transduced cells. A total of 10⁵ freshly purified CD34⁺ cells were transduced and counted 24 hours after transduction in 3 independent experiments. Black bars indicate EV-transduced cells; white bars: Sox6-transduced cells; and exp, experiment. (B) FACS analysis on CD34⁺ transduced cells at different time points after transduction. The x-axis represents days after transduction; y axis, percentage of GFP⁺ cells. Different colors in the histogram columns represent the proportion of GFP⁺ cells that were GpA⁻ (white) or GpA⁺ (black), respectively.