Sox6 enhances erythroid differentiation in cord blood–derived cell cultures. (A top panel) Percentage of GpA⁺ cells, estimated by FACS analysis on different days of the culture. (Bottom panel) Semiquantitative reverse-transcription PCR on the same days. Gels show Sox6 expression (top gel) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH; bottom gel). (B) FACS analysis on erythroblasts transduced either with the EV (EV-erythroblasts) or the Sox6-overexpressing vector (Sox6-erythroblasts). The x-axis represents GFP expression; y-axis, GpA expression. (C) May- Grünwald-Giemsa staining on cytospin preparations of the same samples as above. (D) Differential counts on cells from the same cells as in panel C. More than 200 cells were scored for each sample.