Figure 6
Figure 6. Expression analysis of Prdm16−/− and wt FL HSCs. (A) Scatter dot plots of the expression of the genes, as measured using Fluidigm multiplex quantitative PCR, indicated on top of each panel in 8 groups of 5 Prdm16−/− or wt FL LSKCD150+ HSCs. *P < .002, threshold required after Bonferroni correction. All experimental replicates of the amplification step and the microfluidic quantitative PCR chip are shown. Undetectable expression is defined by a Ct value of 35 or greater. (B) Heat map of the expression levels for Prdm16 and 24 genes shown in panel A.

Expression analysis of Prdm16−/− and wt FL HSCs. (A) Scatter dot plots of the expression of the genes, as measured using Fluidigm multiplex quantitative PCR, indicated on top of each panel in 8 groups of 5 Prdm16−/− or wt FL LSKCD150+ HSCs. *P < .002, threshold required after Bonferroni correction. All experimental replicates of the amplification step and the microfluidic quantitative PCR chip are shown. Undetectable expression is defined by a Ct value of 35 or greater. (B) Heat map of the expression levels for Prdm16 and 24 genes shown in panel A.

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