Effects of Prdm16 on homing, apoptosis, and cell cycling. (A) Homing of Prdm16^−/−, Prdm16^+/−, and wt E16 FL cells after transplantation into lethally irradiated CD45.1⁺ recipients (n = 3). (B) Percentage of FL LSKFlt3⁺ and LSKFlt3⁻ cells from Prdm16^−/−, Prdm16^+/−, and wt mice in S/G₂/M phase of the cell cycle as measured by 4,6-diamidino-2-phenylindole staining. Each experiment contained 2 to 4 embryos per genotype. (C) Fraction of FL LSKFlt3⁺ and LSKFlt3⁻ cells from Prdm16^−/−, Prdm16^+/−, and wt mice in S/G₂/M phase normalized to the values of wt littermate embryos (n = 3 litters). *P < .05. (*)P = .08. (D) Percentage of apoptotic total FL and LSKCD150⁺ FL cells in Prdm16^−/−, Prdm16^+/−, and wt embryos (n = 3 for each genotype). *P = .05, one-way ANOVA.