Functional analysis of T-cell and B-cell responses. Functional responses to antigen are augmented in the primed group. T-cell and B-cell responses to influenza antigens were measured by antigen-specific ELISPOTS. CD4 T-cell antigen–specific responses (γ-interferon responses to intact protein after CD8 T-cell depletion) were increased in the primed group compared with the nonprimed group at the T-cell harvest time point (P = .02). There were no statistically significant differences between the 2 groups for the CD8-specific responses (responses to influenza peptides), and the global responses (phorbol myristate acetate [PMA] and ionomycin and total IgG) also did not differ between the 2 groups at any time point.