E47-deficient bone marrow cells display compromised long-term competitive repopulation activity in vivo. (A) CD45.2 WT and E47 KO bone marrow cells (2 × 105) mixed with an equal number of CD45.1 competitor cells were adoptively transferred into lethally irradiated CD45.1 recipient mice. The proportion of test donor-derived (CD45.2+) Gr-1+cells was monitored every 4 weeks in the peripheral blood of the recipient mice. Then, the recipients were killed at 16 weeks after transplantation, and lineage repopulation in the spleen (Gr-1+, CD11c+) or bone marrow (flk2− LSK) was measured (n = 5 mice). P < .05. The data are representative of 2 independent experiments. (B) Competitive lymphoid repopulation activity of WT and E47 KO bone marrow cells was measured at 1:1 test donor/competitor ratio. The percentage of test donor-derived (CD45.2+) lymphoid cells in the peripheral blood of the recipient mice at 16 weeks after transplantation is shown (n = 8 mice). PB indicates peripheral blood; SPL, spleen; and BM, bone marrow.
